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GRK 1482 Jahrbuch 2011-2014

SENIOR PRINCIPAL INVESTIGATORS Page 14 | GRK Progress Report 2011-2014 Education / Degrees 1972 - 1978 Nutritional Sciences, Universität Giessen, Germany 1978 Diploma in Nutritional Sciences 1982 Ph.D. Biochemistry of Nutrition, ’summa cum laude’ (Univ. Giessen) 1989 Habilitation & venia legendi for Physiology and Biochemistry of Human Nutrition (Universität Giessen) Positions held 1982 - 1989 Research Assoc. & Assistant professor, Universität Giessen, Germany 1982 - 1983 Resident/Fellow, Institute of Physiology, University of Glasgow, UK 1989 - 1992 Research Associate, School of Medicine, University of Pittsburgh/USA 1992 - 1999 Universität Giessen, Professor (C4), Professur für Biochemie der Ernährung 1999 to date Technische Universität München (TUM), Professor (C4), Lehrstuhl für Ernährungsphysiologie 2002 Declined Professor and Director position at the Deutsches Institut für Ernährungsforschung (DIfE) and Universität Postdam. Research Interests and Achievements There are two general themes in the experimental research carried out.One is a gene-guided approach in which membrane nutrient transporters are the main focus. The second line of research targets the interactions of dietary constituents with the genome at the levels of the transcriptome, the prote- ome and the metabolome. As experimental models to assess the structures and functions of transport proteins for amino acids and peptides serve bacteria and yeast but also transgenic C. elegans and mice lines. The spectrum of the research carried out here includes the cloning of the genes, their heterologous expression, site-directed mutagenesis, regulation of gene expression and physiological consequences of a loss of transporter function. In addition to amino acids and peptides, intestinal glucose and fructose transporters are also studied in transgenic mice. In the nutrient guided approaches, profiling techniques such as transcrip- tomics and proteomics but most recently mainly LC-MS/MS based meta- bolite analysis tools are applied. Human studies are conducted to derive biomarkers of exposure and to study the changes in the metabolome fol- lowing food intake in healthy and prediabetic volunteers. As amino acids in plasma have been identified as the most sensitive marker metabolites of impaired insulin sensitivity and type 2 diabetes, animal studies are used as a complement to define the molecular origin of these changes in amino acid metabolism. Prof. Dr. Hannelore Daniel Technische Universität München Physiology of Human Nutrition (Chair) Gregor-Mendel-Str. 2, D-85350 Freising-Weihenstephan Selected Publications Rizzello, A, Romano, A, Kottra G, Acierno R, Storelli C, Verri T, Daniel H, Maffia M. A protein cold adaptation strategy via a unique seven amino acid domain in the icefish (Chionodraco hama- tus) PEPT1 transporter, Proc. Nat. Acad Sci. 2013. Kolodziejczak D, Spanier B, Pais R, Kraiczy J, Stelzl T, Gedrich K, Scherling C, Zietek T, Daniel H. Mice lacking the intestinal peptide transporter display reduced energy intake and a subtle maldigestion/-absorption that protects them from diet-induced obesity. Am J Physiol Gastro- intest Liver Physiol. 2013. Dahlhoff C, Desmarchelier C, Sailer M, Fürst RW, Haag A, Ulbrich SE, Hummel B, Obeid R, Geisel J, Bader BL, Daniel H. Hepatic Methionine Homeo- stasis Is Conserved in C57BL/6N Mice on High- Fat Diet Despite Major Changes in Hepatic One- Carbon Metabolism. PLoS One. 2013, 8(3). Krug S, et al., Daniel H. The dynamic range of the human metabolome revealed by challenges. FASEB J. 2012, 26(6):2607-19. Research Goals Understand nutrient transport processes for sugars, amino acids and peptides in epithe- lia at the structural and functional level Assess the role of intestinal peptide trans- porters as targets for drug delivery Define the molecular and cellular processes of how nutrients participate in control of gene expression Apply transcriptomics, proteomics and metabolite profiling as biomarker discovery tools