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GRK 1482 Jahrbuch 2011-2014

Publications [1] WHO. Overweight, Situation and trends. 2008. [2] Mensink G, Schienkiewitz A, Scheidt-Nave C. DEGS Symposium. 2012. [3] Puhl RM, Heuer CA. The stigma of obesity; a review and update. Obesity 2009, 17: 941-964. [4] Flegal KM, Graubard BI, Williamson DF, Gail MH. Excess deaths associated with underweight, overweight, and obesity. JAMA 2005, 293:1861-1867. [5] Pi-Sunyer FX. Comorbidities of overweight and obesity current evidence and research issues. Med Sci Sports Exerc. 1999, 31:602-608. [6] Xu H, Barnes GT, Yang Q, Tan G, Yang D, Cou CJ, Sole J, Nichols A, Ross JS, Tartaglia LA, Chen H. Chronic in- flammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest. 2003, 112:1821-1830. [7] KeophiphathM,RouaultC,DivouxA,ClementK,LacasaD. CCL5 promotes macrophage recruitment and survival in human adipose tissue. Arterioscle Thromb Vasc Biol. 2010, 30:39-45. [8] Pais R, Zietek T, Hauner H, Daniel H, Skurk T. RANTES (CCL5) reduces glucose-dependent GLP-1 secretion from intestinal L-cells. Submitted. [9] Williamson DF, Pamuk E. Thun M, Flanders D, Byers T, Heath C. Prospective study of intentional weight loss and mortality in never-smoking overweight US white women aged 40-64 years. Am J Epidemiol. 1995, 141: 1128-1141. [10] Maggard MA, Shugarman LR, Suttorp M, Maglione M, Sugerman HJ, Livingston EH, Nguyen NT, Li Z, Mojica WA, Hilton L, Rhodes S, Morton SC, Shekelle PG. Meta-analysis: surgical treatment of obesity. Ann Intern Med. 2005, 142: 547-559. ASSOCIATED FELLOWS GRK Progress Report 2011-2014 | Page 65 Aim Our aim is to investigate the influence of bariatric surgery, in particular LGS, on inflammation in adipose tissue as well as on changes in the secretion of adipokines, like RANTES. Further, we want to take a closer look on complications of this surgical procedure, such as vitamin absorption. Methods and Results In collaboration with the surgical clinic Mu-nich-Bogenhausen, obese patients who are undergoing LGS are recruited. Blood samples as well as adipose tissue samples (visceral and sub- cutaneous) are taken at the day of operation. Adipose tissue is snap-frozen on dry-ice for RNA-, protein- and DNA-isolation and a small piece is fixed in 4 % buffered formalin for histologi- cal investigations. Adipokines are measured in blood samples before and after surgery, with enzyme-linked immunosorbent assay. Hence, mRNA-Levels of adipokines are determined in adipose tissue via reverse transcription polymerase chain reaction. Immuno- histochemnistry is performed to measure infiltration of adipose tissue by macrophages in histological slides During the first year after surgery, patients will be invited for evaluation and a dietary protocol is requested from the patient. Furthermore, blood will be drawn every three months to assess vitamin status and inflammatory parameters. Outlook To assess the degree of tissue inflammation the phenotype of macrophages and other infiltrating leukocytes in adipose tis- sue will be determined The grade of tissue inflammation will be correlated to immune factors in the collected blood samples. In addition, with the use of dietary protocols before and after surgery we will evaluated the vitamin status of the patients. After major weight loss, most patients are undergoing abdomi- nal plastic surgery, which enables further investigations of the adipose tissue after weight loss. Figure: Hematoxylin and eosin stained histological slice of subcutaneous adipose tissue from a subject with Body Mass Index of 63 kg/m² Supervisors PD Dr. Thomas Skurk I TUM I Nutritional Medicine Prof. Dr. Matthias Blüher I University Hospital Leipzig I Department for Internal Medicine Start of project: September 2012 Academic background: Studies of Nutritional Science at Justus-Liebig-Universität Gießen